Abedi, 28, is accused of attacking four prison officers with hot oil and makeshift weapons in a suspected ambush at HMP Frankland in County Durham on 12 April.
He appeared at the Old Bailey by video-link from Belmarsh prison, southeast London, wearing a grey tracksuit.
Sat at a desk in handcuffs, Abedi was surrounded by five prison officers wearing body armour and helmets with face shields.
Abedi pleaded not guilty to three counts of attempted murder, relating to two male prison officers and one female prison officer.
He also denied one charge of assault occasioning actual bodily harm and one count of having unauthorised offensive weapons inside prison.
Three prison officers were taken to hospital with serious injuries after the alleged ambush.
At the time of the incident, the Prison Officers Association said the staff sustained life-threatening injuries including burns, scalds and stab wounds.
During a hearing on Friday, Abedi spoke to confirm his name and date of birth and told the judge Mrs Justice Cheema-Grubb he did not want to be represented at his trial, which is listed to start on 18 January 2027.
Abedi was convicted of assisting with the Manchester terror plot, in which his brother, suicide bomber Salman Abedi, killed 22 people by detonating a homemade rucksack bomb among a crowd of concert-goers.
He was jailed for life with a record-breaking 55-year minimum term in August 2020.
