The 36-year-old pleaded not guilty to assault occasioning actual bodily harm (ABH) to music producer Abraham Diaw, during a hearing at Southwark Crown Court on Friday.
Brown also denied one count of having an offensive weapon – a bottle – in a public place.
The Grammy-winning US musician last month pleaded not guilty to a more serious charge of attempting to cause grievous bodily harm (GBH) with intent to Mr Diaw.
The attack allegedly happened at the Tape venue, a private members’ club in Hanover Square, Mayfair, on 19 February 2023.
The plea hearing is part of preparations for his five to seven-day trial, which is due to take place from 26 October 2026.
Brown’s co-defendant, US national Omololu Akinlolu, 39, on Friday pleaded not guilty to a charge of assaulting Mr Diaw occasioning him actual bodily harm.
Akinlolu, a rapper who goes by the name Hoody Baby, has previously pleaded not guilty to attempting to cause grievous bodily harm.
The defendants sat side-by-side in the dock, looking straight ahead during the hearing in London.
Around 20 fans sat in the public gallery behind the dock for Friday’s hearing, with several gasping as Brown walked into the courtroom.
The Go Crazy singer was able to continue with his scheduled international tour after he was freed on conditional bail in May.
He had to pay a £5m security fee to the court as part of the bail agreement, which is a financial guarantee to ensure a defendant returns to court and may be forfeited if they breach bail conditions.
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Mr Diaw was standing at the bar of the Tape nightclub when he was struck several times with a bottle, and then pursued to a separate area of the venue where he was punched and kicked repeatedly, Manchester Magistrates’ Court previously heard.
Brown was arrested at Manchester’s Lowry Hotel at 2am on 15 May by detectives from the Metropolitan Police.
He is said to have flown into Manchester Airport on a private jet in preparation for the UK tour dates.
Brown was released from HMP Forest Bank in Salford, Greater Manchester, on 21 May.
The singer, who rose to stardom as a teenager in 2005, won his first Grammy award for best R&B album in 2011 for F.A.M.E..
He earned his second in the same category for 11:11 (Deluxe) earlier this year.
